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Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells (including cells in the culture supernatant)
Pycard Immunogen Recombinant Protein Location Cytoplasm
including the iC3b fragment of complement protein
Pepsin is an endopeptidase that breaks down proteins into smaller peptides
including Kupffer cells
Mouse ANGPT1 ELISA Kit Size:96T Centrifuge washing with pre-cooled PBSProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
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